中国媒介生物学及控制杂志

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云南省大理市鼠、蜱中检测出斑点热群立克次体DNA

张海林1;杨红2;张泮河2;曹务春2;张云智1;袁庆虹1;杨卫红1;王静林1;米竹青1   

  1. 1云南省地方病防治所病毒立克次体室 大理671000;2军事医学科学院微生物流行病研究所
  • 出版日期:2004-12-20 发布日期:2004-12-20

Spotted Fever Group Rickettsia DNA was Detected in Wild Rodent and Tick in Dali, Yunnan Province, China

ZHANG Hai-lin*; YANG Hong; ZHANG Pan-he; et al   

  1. Yunnan Provincial Institute of Endemic Disease Control and Prevention, Dali 671000, China
  • Online:2004-12-20 Published:2004-12-20

摘要: 目的了解鼠、蜱中自然感染斑点热群立克次体状况。方法用PCR法检测鼠类脾脏和蜱类中的斑点热群立克次体DNA序列片段。结果在云南省大理市下关野外山地捕获鼠类9种51只,从大足鼠(1/15)、大绒鼠(1/7)和短尾(1/8)各检出阳性1份,其他鼠种均为阴性,总阳性率为5.88%。从采自弥渡县的208只勐突血蜱中检出阳性5份,阳性率为2.40%。结论初步认为云南省大理地区存在斑点热疫源地。

关键词: 鼠类, 蜱类, 斑点热群立克次体, 聚合酶链反应

Abstract: Objective To study the infection of Spotted Fever Group Rickettsia(SFGR) in wild rodents and ticks in Dali,Yunnan province.Methods Detecting SFGR DNA sequence fragment in spleen of rodents and ticks by PCR assay.Results The 51 rodents belonging to 9 species were captured in Xiaguan,Dali city.Three specimens were positive in spleen of rodents that were Rattus nitidus(1/15),Eothenomys miletus(1/7) and Anowrosorex squamipesi(1/8).Five specimens were positive in 208 ticks(Haemaphysalis montgomeryi) collected in Midu county,Dali prefecture.Conclusion It shows preliminarily that there is natural focus of Spotted Fever in Dali,Yunnan province.