关键词: 恙虫病, 血清学, 间接免疫荧光法

Abstract: Objective:To obtain Rickettsia tsutsugamushi(R.t.)antibody level in patients and healthy population of epidemic area and non-epidemic area,and to get the seroepidemiological pattern of tsutsugamushi disease in Hebei province based on serological typing and dynamic observation.Methods:Serum specimens of different time series after the onset of tsutsugamushi disease were collected from patients.Serum specimens were also collected from healthy population in epidemic area and non-epidemic area.Indirect immunofluorescence were carried out to detect Rt antibody and to identify the serological type.Results:Gilliam serological type was identified in all of the 27 patients.(R.t.) IgG and IgM antibodies could be detected at 3 days after the onset of disease.And positive rate of both Rt IgG and IgM antibodies were 100% from 20 days to 1 year after the onset of disease.The titers of antibodies reached the peak point at 10-19 days after the onset of disease,and then declined gradually.Positive rates of (R.t.) IgG and IgM were 7.70% and 4.81% in healthy population of epidemic area,respectively.And the antibodies were not detected in healthy population of non-epidemic area.Conclusion:Seroepidemiological pattern of tsutsugamushi disease was clarified primarily.In epidemic area,recessive infection might exist.